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Promax, Efficacy of Promax on Strawberry Pathogens, Lab Report

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Page 1 1331 W. Houston Avenue, Gilbert, AZ 85233 | 800.961.1220 | Fax 480.425.3061 | | ©2017, Trademarks and registered trademarks are property of Bio Huma Netics, Inc. In Vitro Bio-Assay Testing of PROMAX ® Efficacy in Controlling Strawberry Pathogens Laboratory Report Plant Sciences, Inc. Objective Test the efficacy of Promax ® for inhibiting mycelial growth of 8 fungal strawberry pathogens through in vitro bio-assay. Methods Potato dextrose agar was amended with Promax ® at a rate of 2% after autoclaving and cooling to 55°C on a stir plate. The amended media was poured into Petri plates and, once cooled and solidified, they were inoculated with 8 economically important strawberry pathogens (see list, next column). Plates were inoculated by placing a 5 mm mycelial agar plug, taken from actively growing culture, onto the center of the amended media. Non-amended agar plates were also inoculated as a negative control treatment. For each treatment by pathogen combination, three replicate plates were inoculated. The plates were incubated at 20°C for 2 weeks. The diameter of each mycelial colony was measured weekly. The % inhibition by the test chemical was calculated using the difference between the mean of replicates in the negative control group and the treated group. The following 8 fungi and fungal-like pathogens were tested: • Botrytis cinerea • Colletotrichum acutatum • Cylindrocarpon destructans • Fusarium oxysporum f. sp. fragariae • Macrophomina phaseolina • Phytophthora ramorum • Rhizoctonia solani • Verticillium dahliae Results After 1 week of incubation, all 8 pathogens tested were completely inhibited from mycelial growth in media amended with 2% Promax ® (see photos, pages 2 and 3). After 2 weeks, 7 of the 8 pathogens were still 100% inhibited. Verticillium dahliae began to grow a little after 2 weeks; the mean percentage inhibition of V. dahliae was 94% after 2 weeks (Figure 1). 0% 20% 40% 60% 80% 100% % inhibition of mycelial growth Pathogen genera 1 week incubation 2 week incubation Botrytis Colletotrichum Cylindrocarpon Fusarium Macrophomina Phytophthora Rhizoctonia Verticillium Figure 1. % Inhibition of Mycelial Growth of 8 Strawberry Pathogens In Vitro Using Agar-Based Media Amended With 2% Promax ® HG-170315-01

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